RESEARCH ARTICLE


Investigating Micronucleus Changes in Mouse Lymphocytes Due to Exposure to Silica Dust



Farideh Golbabaei1, Abdollah Gholami2, *, Gholamheidar Teimori-Boghsani3, Mojtaba Kianmehr4, Mehdi Yaseri5
1 Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2 Department of Occupational Health Engineering, School of Public Health, Birjand University of Medical Sciences, Birjand, Iran
3 Department of Environmental Health, School of Health, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
4 Department of Basic Sciences, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
5 Department of Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


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© 2019 Golbabaei et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Occupational Health Engineering, School of Public Health, Birjand University of Medical Sciences, Birjand, Iran; Tel: + 989907514341;E-mail: gholamiabdollah@yahoo.com


Abstract

Background and Objectives:

Occupational exposure to silica dust has multiple consequences, including genetic complications. One of the genetic complications is Micronucleus (MN) changes; therefore, this study aims to evaluate the rate of MN formation in mouse lymphocyte cells due to exposure to silica dust.

Materials and Methods:

Totally 72 male mice BALB/c were selected and categorized into five exposure groups with 12 mice in exposure to the concentrations of 1.3, 3, 8, 12, and 17 mg/m3 of 99% pure silica dust and a control group. The mice were exposed to silica dust in which they were exposed for 8 hours a day, 6 days a week, and for 1, 2, 3, and 4 months. Then, blood samples were taken from the mice and the rate of MN formation in their lymphocyte cells was evaluated. The results were analyzed via SPSS software version 21 (P<0.05).

Results:

Maximum and minimum averages of dust concentration, related to boxes 1 and 5, were 17 mg/m3 and 1.3 mg/m3, respectively. Maximum rate of MN formation was observed in the fourth month of exposure and in group 1 with the value of 21.6±1.15, and minimum rate of MN formation was observed in the third month of exposure and in control group with the value of 3±1. Average of MN frequencies in each of the exposure month was significant related to the control group (P=0.001). There was a direct and significant correlation between exposure concentrations of exposed group and average rate of MN formation (r=0.679).

Conclusions:

More than 3 months exposure to silica dust may lead to significant MN formation in lymphocytes of mice BALB/c in comparison with the control group.

Keywords: Dust concentration, BALB/c, Lymphocytes, Micronucleus, Mouse, Silica dust.