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Methods for Separate Isolation of Cell-Free DNA and Cellular DNA from Urine-Application of Methylation-Specific PCR on both DNA Fractions
Agnes Beermann, 1
Foued Ghanjati, 1
Thomas Hermanns2
Cedric Poyet2
Joana Pereira1
Johannes Fischer1
Peter Wernet1
Simeon Santourlidis, *
, 1
Authors Info & Affiliations
, 1
Authors Info & Affiliations
Abstract
The analysis of genomic DNA is widely-used for research, forensic and diagnostic purposes. Here we describe reliable methods for isolation of cell-free DNA and cellular DNA from urine. Both DNA fractions are suitable for PCR and Methylation-Specific PCR (MSP) amplification, leading to consistent and reproducible results. A kinetics analysis describes the decline of efficiency of MSP performed with urinary DNA which had been stored at room temperature with and without proteinase K for various time periods.
Keywords: Cell-free DNA, Diagnosis, DNA methylation, Urine.
