COL1A1 Gene Expression in Hepatitis B Virus (HBV) Related Hepatocellular Carcinoma (HCC) Egyptian's Patients

Amal A. Mohamed1, Yousry Esam-Eldin Abo-Amer2, Amyan Aalkhalegy3, Lamiaa Abdelfattah Fathalla4, Mostafa Bedair Elmaghraby5, Mohamed Mahmoud Elhoseeny6, Sahar Mohamed Mostafa7, Mohamed El-Abgeegy7, Rania Abdelmonem Khattab8, Dalia Ali El-damasy9, Wafaa Salah10, Abeer Mohammed Salem11, Wael Mohamed Elmashad12, Mohamed Elbahnasawy13, Sherief Abd-Elsalam14, *
1 Department of Biochemistry, National Hepatology Tropical Medicine Research Institute, Cairo, Egypt
2 Department of Gastroenterology and Infectious Diseases, Hepatology, Mahala Hepatology Teaching Hospital, Gharbiya, Egypt
3 Department of Surgery, Amyan Aalkhalegy, Al Sahel Teaching hospital, Cairo, Egypt
4 Clinical Pathology, National Cancer Institute, Cairo University, Giza, Egypt
5 Department of Gastrointestinal and Hepatobiliary Surgery, Mahala Hepatology Teaching Hospital, Gharbiya, Egypt
6 Mahalla Teaching Liver Hospital, Gharbiya, Egypt
7 Department of Liver Transplantation, National Hepatology and Tropical Medicine Research Inistitue, Cairo, Egypt
8 Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt
9 Department of Microbioogy, Faculty of Pharmacy, Egyptian Russian University, Badr City, Egypt
10 Department of Internal Medicine, National Institute of Diabetes and Endocrinology, Cairo, Egypt
11 Department of Internal Medicine, KasrAlainy, Faculty of Medicine, Cairo University, Cairo, Egypt
12 Department of Physiology, Tanta University, Tanta, Egypt
13 Department of Emergency Medicine and Traumatology, Tanta University, Tanta, Egypt
14 Department of Tropical Medicine and Infectious Diseases, Tanta University, Tanta, Egypt

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© 2021 Mohamed et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Tropical Medicine and Infectious Diseases, Faculty of Medicine, Tanta University, El-Giash Street 31527, Tanta, Egypt; Tel: +2-01224608774; E-mail:



Collagens are the most abundant proteins in the human body, accounting for one-third of total proteins. Over the last few years, accumulated evidence have indicated that some collagens are differentially expressed in cancer. The aim of the study was to assess COL1A1 gene expression as a novel marker for the progression of hepatitis B cirrhosis into hepatocellular carcinoma.


This cohort study included 348 subjects and was conducted between May 2018 and June 2019. Subjects were divided into 4 groups: group1 included HBV positive hepatocellular carcinoma patients “HCC” (n= 87), group II included HBV positive patients with liver cirrhosis “LC” (n = 87), group III included chronic hepatitis B patients with neither HCC nor cirrhosis “ C-HBV” (n = 87) and group IV consisted of healthy volunteers as controls (n = 87). Fasting venous blood samples (10 ml) were collected from each participant in this study and were used for assessment of aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin, albumin and alfa-fetoprotein (AFP). Another portion of blood was collected in 2 vacutainer tubes containing EDTA, one for Complete blood count and the other for gene expression of COL1A1.


The gene expression of collagen was 6.9 ± 8.8 in group 1 (HBV positive hepatocellular carcinoma patients) and this was a significant increase in comparison with the other groups. In group 2 (HBV positive patients with liver cirrhosis), the gene expression (collagen) was 3.7±1.5 and it was significantly increased when compared with group 4 (healthy volunteers).


COL1A1 gene expression can be used as an indicator of the progression of hepatitis B cirrhosis into hepatocellular carcinoma.

Keywords: Hepatocellular carcinoma, HCC, HBV, Collagen, Gene, Cirrhosis.