The Effect of n-6 Polyunsaturated Fatty Acid on Blood Levels of Malondialdehyde-Deoxyguanosine Adducts in Human Subjects



S.A. Moore*, 1, 2, E. Humphreys3, M.D. Friesen4, D.E.G. Shuker 2, S.A. Bingham 3
1 School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, UK
2 Department of Chemistry and Analytical Sciences, The Open University, Walton Hall, Milton Keynes, UK
3 Dunn Human Nutrition Unit, Wellcome Trust/Medical Research Council Building, Cambridge, UK
4 IARC, Lyon, France; Current address: Johns Hopkins Bloomberg School of Public Health, Department of Environmental Health Sciences, Baltimore, MD, USA


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© 2008 Moore et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, UK; Tel: 0044 (0) 151 231 2049; Fax: 0044 (0) 151 231 2170; E-mail: s.a.moore@ljmu.ac.uk


Abstract

The role of n-6 polyunsaturated fats upon the formation of the mutagenic DNA adduct malondialdehydedeoxyguanosine (M1dG) in blood was investigated in male volunteers (n = 13) who consumed diets high in saturated and polyunsaturated fats, and polyunsaturated fat plus a-tocopherol supplemention (400 IU per day). On day 14 there was a significant difference in adduct levels between diets with saturated fats giving higher levels than polyunsaturated fats but this effect had disappeared by day 20 indicating that there is a relatively rapid adjustment to the effects on DNA damage of changes in dietary fat. a-Tocopherol showed a small benefit by day 20. Five females participated in the PUFA study and had higher mean adduct levels than men but there was no correlation with hormonal status. Overall, PUFA had a limited beneficial effect on M1dG levels that warrants further investigation.