RESEARCH ARTICLE


Epidermal Growth Factor rs4444903 A>G Gene Polymorphism Association with Chronic Hepatitis B Liver Disease Progression among Egyptian Children: A Multicenter Study



Amal A. Mohamed1, Gehan L.A. Hakeem2, Gihan M. Babrs2, Laila E. Abolfotoh2, Nageh M. Shehata2, Sheren E. Maher2, Suzan M. Mousa2, Ahlam M. Ismail3, Doaa Ezzat4, Ahmed G.K. Habib5, Doaa M. Ghaith6, Naglaa Fawazy7, Rania A. Khattab8, Eman El S. Habib9, Dalia M. Abd El-Hassib10, Sherief Abd-Elsalam11, *, Dalia A. El-damasy12
1 Department of Biochemistry, National Hepatology and Tropical Medicine Research Institute, Cairo University, Cairo, Egypt
2 Department of Pediatrics, Faculty of Medicine, Minia University, Minia, Egypt
3 Department of Pediatrics, Faculty of Medicine, South Valley University, Qena, Egypt
4 Department of Biochemistry, Misr University for Science and Technology, Giza, Egypt
5 Department of Biotechnology and Life Science, Faculty of Postgraduate Studies for Advanced Sciences, Beni- Suef University, Beni-Suef, Egypt
6 Department of Clinical and Chemical Pathology, Faculty of Medicine Cairo University, Cairo, Egypt
7 Department of Clinical and Chemical Pathology, National Institute of Diabetes and Endocrinology, Cairo, Egypt
8 Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt
9 Department of Clinical Nutrition, National Nutrition Institute, Cairo, Egypt.
10 Department of Clinical and Chemical Pathology, Benha Faculty of Medicine, Benha University, Qaluybia, Egypt
11 Department of Tropical Medicine, Tanta University, Tanta, Egypt.
12 Department of Microbiology, Faculty of Pharmacy, Egyptian Russian University, Badr City, Egypt

Article Information


Identifiers and Pagination:

Year: 2021
Volume: 11
First Page: 63
Last Page: 68
Publisher ID: TOBIOMJ-11-63
DOI: 10.2174/1875318302111010063

Article History:

Received Date: 20/1/2021
Revision Received Date: 30/3/2021
Acceptance Date: 28/4/2021
Electronic publication date: 12/07/2021
Collection year: 2021

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Creative Commons License
© 2021 Mohamed et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at Department of Tropical Medicine and Infectious Diseases, Faculty of Medicine, Tanta University, El-Giash Street 31527, Tanta, Egypt, Telephone: +2-01063319696- E-mail: sherif.abdelbaky@med.tanta.edu.eg


Abstract

Background:

Polymorphisms of genes encoding the pro-inflammatory and anti-inflammatory cytokines can affect the clinical presentation of the infection. We aimed to assess the role of EGF gene single-nucleotide polymorphism in the outcome of chronic hepatitis B virus (HBV) infection in children.

Methods:

One hundred HBV-infected children and 75 healthy matched controls were enrolled in this prospective study. Patients included 18 chronic inactive and 82 chronic active carriers. EGF rs4444903 A>G genotypes were determined using allele-specific amplification.

Results:

Significant differences regarding EGF genotypic frequency (p=0.001) in patients compared to controls (p=0.001). Eighteen percent were inactive, and 82% were active carriers. AA, AG and GG genotypic frequency were 66.7%, 33.3%, 0% and were 3.7%, 37.8% and 58.5% in the inactive and active carriers, respectively, with significant differences regarding AA, AG, GG genotypic frequency (p=0.001 for all). EGF AA, AG, GG genotypes frequency were 1.9%, 33.3%, and 64.8%, respectively, with significant differences between cirrhotic and non-cirrhotic patients regarding AA, AG, GG genotypic frequency (p=0.001 for all).

Conclusion:

Increased G allele frequency in EGF rs4444903 A > G polymorphism in HBV- Egyptian children is associated with worse outcomes.

Keywords: HBV, Carriers , Cirrhosis , EGF , Polymorphism , Egyptian children .