Protective Efficacy of Vitamin F against Acrylamide Induced Toxicity: Studies on Oxidative Stress Biomarkers

RESEARCH ARTICLE Protective Efficacy of Vitamin F against Acrylamide Induced Toxicity: Studies on Oxidative Stress Biomarkers Sadhana Shrivastava, Satendra K. Nirala, Mohammad S. Reshi, Sangeeta Shukla, Anjali Sharma and Chhavi Uthra UNESCO Satellite Centre for Trace Element Research UGC-SAP School of Studies in Zoology, Jiwaji University, Gwalior (M.P.) India Department of Rural Technology and Social Development Guru Ghasidas (Central) University, Bilaspur (C.G.), India


INTRODUCTION
Nowadays, there is an increased interest in the function and medicinal properties of specific dietary fatty acids.Linoleic Acid (LA) is a metabolic precursor for arachidonic acid synthesis and essential fatty acids in the human diet [1,2].It phenols which inhibit cholesterol and fatty acid biosynthesis pathway [1].LA is an essential omega-6 polyunsaturated fatty acid with 18-carbon chain with two sites of unsaturation (Fig. 1).Acrylamide (AA) is a vinyl monomer having high watersolubility.It is used in the manufacturing polyacrylamides, in cosmetic industries such as lotions, cosmetics, deodorants [7].Recently, it was detected in certain food products which raises a worldwide concern because nowadays every individual exposed to AA contained food products such as coffee, cookies, bread, potato crisps [8,9].AA is produced when carbohydrate-rich foods are processed at high temperature (higher than 120°C) during baking, grilling or frying [10].It is carcinogenic, nephrotoxic, neurotoxic and induces reproductive toxicity exposure in mice and rats [11,12].Children eat more AA containing food than adults because they consume high AA rich diet and high calories relative to body weight [13].AA is known as a potent neurotoxic agent damaged the peripheral and central nervous system.The neurotoxic effect includes muscle weakness, paraesthesia, foot drop, the absence of tendon reflex, neurotrauma and muscular atrophy due to oxidative stress [14].Recently, growing evidence on the therapeutic efficacy of Linoleic Acid (LA), this study investigates its protective effect against AA toxicity.

Experimental Animals
Female albino rats of Wistar strain (160±10 g body wt) were used in this study.Animals were housed under standard husbandry conditions (25±2°C temp, 60-70% relative humidity and 12 h photoperiod) and had access to standard rat feed and drinking water ad libitum.Animals were treated and cared in accordance with the guidelines recommended by the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA)

Chemicals
Acrylamide and linoleic acid were purchased from Sigma Aldrich.All other chemicals and reagents were procured from Ranbaxy, New Delhi and Himedia Laboratories Ltd. Mumbai, India and are of analytical grade.All diagnostic kits were procured from E-Merck, autoanalyzer (Micro Lab 200, Merck) was used for the measurements.All animals were sacrificed after 24 h of last treatment.

Biochemical Assays
Blood was collected from retro-orbital venous sinus and was allowed to stand at room temperature for 30 min and serum was harvested by centrifugation at 2000 rpm for15 min [15].Serum was used for the determination of aspartate aminotransaminases and alanine aminotransaminases [16].Autoanalyser was used to determine the concentration of Triglyceride (TG), cholesterol, creatinine,albumin and urea in serum (kit method).Brain Acetylcholinesterase (AChE) activity was also assessed [17].Levels of LPO [18], GSH [19], Catalase [20] and Superoxide dismutase [21] were estimated in liver, kidney and brain.

Histopathology
Immediately after necropsy, hepatic, renal and cerebral tissues were excised out and washed in normal saline and immediately fixed in Bouin's fixative.Paraffin sections were routinely prepared at 5μm thickness.Hematoxylin and Eosin (H&E) staining was done and stained slides were observed under a light microscope.

Statistical Analysis
The Standard Deviation (SD) measures the amount of variability or dispersion for a subject set of data from the mean whereas, Standard Error of Mean (SEM) measures how far the sample mean of the data is likely to be from the true population.Thus, SEM is more appropriate for data of small sample size and the results have been presented as Mean ± SEM.Further, significant between two groups were measured by Student's t test [22] followed by ANOVA instead of hypothetical probability value.

Biochemical Results
Tables 1-3 depicts that there was significant (P≤0.05)elevation in the serum transaminases (AST and ALT) activity and bilirubin, whereas depletion in the haemoglobin percentage after AA administration.Therapy with LAat 5,10,20,40 mg/Kg doses restored the levels of serum transaminases, haemoglobin percentage, bilirubin and albumin (Tables 1 & 3).The toxic effect of AA was significantly attenuated by treatment with LA at 20,40 mg/Kg compared with 5,10 mg/Kg dose (p ≤ 0.05).
Table 2 demonstrated that AA intoxication caused a significant (P≤0.05)elevation in serum lipid profile such as triglycerides, cholesterol.Also, exposure to AA caused a O HO significant increase in the concentration of urea, creatinine in serum (P≤0.05) as compared to the control group due to kidney dysfunctions.Therapy with LA at 5,10,20,40 mg/Kg doses prevented all the parameters and recovered in a statistically significant manner (Table 2).
Acetylcholinesterase (AChE) activity in rat brain was evaluated, and the results are shown in Table 3. Brain acetylcholinesterase (AChE) activities were significantly inhibited (P≤ 0.05) after AA exposure.When treated with LA at different dose levels (5,10,20,40 mg/Kg) significantly ameliorated the activity of AChE (P≤0.05)(Table 3).
The alterations in the level of TBARS and GSH are shown in Table 4.The production of TBARS increased in liver, kidney and brain tissues with AA exposure (P< 0.05), whereas GSH levels were significantly (P ≤ 0.05) decreased in AA group as compared to control and LA per se groups.Treatment with therapeutic agent LA (5,10,20,40 mg/Kg) significantly decrease the formation of TBARS and enhanced the GSH contents in all tissues (P < 0.05).Table 5 shows the hepatic, renal and cerebral SOD and CAT activities.Compared with the control group, administration of AA alone significantly declined the SOD and CAT activity (P≤0.05).Therapy with LA (5, 10, 20, 40 mg/Kg), SOD and CAT activity was effectively restored in all the organs (Table 5).
Data of this study showed no significant difference between the activity of ALT, AST, SOD, CAT, AchE as well as the levels of bilirubin, urea, creatinine, GSH, TBARS of the control group and LA per se (40 mg/kg) group.These results suggest that 20mg/kg dose of LA is the effective dose for preventing AA-induced oxidative stress in rats.

Histological Results
Liver of control rat shows the normal central vein, portal area and sinusoidal spaces with the surrounding hepatocytes.Following 10 days of AA administration, disruption in the hepatic cord, Kupffer cells proliferation, heterochromatic nuclei, were detected in between the hypertrophied and vacuolated cytoplasm of hepatocyte (Fig. 3).Therapy with the LA group showed improvement in hepatic cords with maintained Kupffer cells, nuclei, the central vein with almost hexagonal hepatocytes (Fig. 4).distortion in the endothelial lining of Bowman's capsule, hypertrophied epithelial cells of proximal convoluted tubule (Fig. 5).Treatment with both the higher doses of LA revealed compact glomeruli with well-formed Bowman's capsule, epithelial cells of tubules and intact endothelial lining (Fig. 6).
Brain sections of control rats showed well-formed cerebral cortex.AA induced vacuolization, degenerated pyramidal cells and reduced nerve fibres in the cerebrum (Fig. 7).Treatments of LA at 20, 40 mg/Kg showed signs of recovery such as loss of vacuolization with well formedPurkinje and pyramidal cells.Both the higher doses showed an almost equal percentage of protection (Fig. 8).

DISCUSSION
Humans are exposed to acrylamide from various sources including industrial products, cigarette smoking and research laboratories [23].Acrylamide is used in water treatment and grouting in industries and in polyacrylamide gel electrophoresis in research laboratories.AA is readily absorbed in the gastrointestinal tract after ingestion and distributed throughout the body such as the thymus, liver, heart, brain, kidneys in animals and humans.AA is metabolized via two competing pathways: the first formation of glycidamide (GA) by epoxidation reaction mediated by cytochrome P450 2E1 and the second via GSH conjugation [24,25].The CYP2E1 is involved in the conversion of AA to GA in humans and has been shown in both in vivo and in vitro and can bind in vivo with hemoglobin, serum albumins, DNA and enzymes [26,27].In comparison to acrylamide, glycidamide is more reactive towards proteins and DNA.It can be further hydrolyzed by an epoxide hydrolase to less reactive water-soluble 2,3-dihydroxypropionamide (glyceramide) [28,29].
The doses of acrylamide administered to the animals in this study were very toxic that severely affected the muscular system and blood variables of the experimental animals.These parameters could also hamper the metabolism of liver, kidney and brain and physiology of many organs.However, the results of the entire experiment have been described as Mean ± SEM of the small sample size of the experiment to show the variation among data of various groups The Haemoglobin (Hb) is dependent on the red blood cells population.The results of this study have shown that AA administration significantly declined Hb percentage in rats.This may be due to the delayed synthesis of Hb or haemoglobindestruction [23].Reactive Oxygen Species (ROS) occurring at a certain level during normal metabolism is detoxified by antioxidant systems of the cell and this prevents from oxidative damage that may occur in the macromolecules of the cell.Oxidant/antioxidant balance is the relation between the level of oxidants occurring in the cell under physiologic conditions and total antioxidant capacity of the cell [30].GSH takes charge in the antioxidant defense system of the cell.The active group of glutathione is a thiol (-SH) group in cysteine residue.It is synthesized and present in all mammalian cells.AA administration increases the generation of free oxygen radicals and decreases the GSH in all tissues of experimental animals because more GSH is used for detoxification.LA in accordance with the high GSH level within the cell decreased the GA generation and prevented the genotoxic damage based on glycidamide in a significant manner.Therefore, oxidative damage occurs in cells due to spoiling oxidant/antioxidant balance [31].ROS generation causes oxidative deterioration of lipids resulting in lipid peroxidation.Our study found that the level of thiobarbituric acid reactive products had increased significantly in liver, kidney and brain after administrated with AA due to the failure to detoxify the AA.Similar results noted by many researchers [8,14,32].
A significant increment in AST and ALT activity was observed in the serum of AA exposed rats.These enzymes are originated from the cytoplasm.After exposure to toxicants or suffering from liver malfunctioning, these enzymes ooze into the bloodstream, thereby making cell membrane permeable.A similar finding was observed by many workers [14, 33 -36].Data of the current study indicate a significant decline in the albumin level after AA administration due to changes in protein metabolism in the liver or retarded protein synthesis [33].AA also caused liver necrosis, cytoplasmic fatty vacuolation and lymphocytic infiltration [35].Data of this investigation showed that AA cause increment in the serum creatinine and urea as compared to a control group which might be due to impairment in renal function [33,37] Superoxide Dismutase (SOD) and Catalase (CAT) are antioxidant enzymes.These enzymes protect cells against ROS induced damage.Therefore, these enzymes have been used to determine oxidative stress in cells [38].AA induced toxicity which may be due to the decrease of antioxidant defense system enzymes or induce oxidative stress with the loss of free radical scavenging activity.So, AA depletes the hepatic, renal and cerebral SOD and CAT activities thus induced oxidative stress.This suggests more utilization of these antioxidant enzymes with the consequent decline to counter the increased level of ROS by AA in liver, kidney and brain.Similar findings were observed by many authors [34, 39 -41].LA at different doses can restore the activity of antioxidant enzymes and possibly reduce the generation of free radicals.
AA is a potent neurotoxic agent.It causes central and peripheral neuropathy in experimental animals.Acetylcholinesterase is presynaptic and postsynaptic components of these pathways where AChE terminates the neurotransmitter's (acetylcholine) synaptic action through catalytic hydrolysis.In the present investigation, significant down regulation in the brain Acetylcholinesterase (AChE) activity was observed which may be due to impaired function of synapses.This involves the adduction of presynaptic protein thiol groups and ultimately impaired synaptic neurotrans-mission or associated to decrease in liver GSH level.Similar results found by several authors [32, 42 -44].
Our biochemical findings were supported by our histological observations.AA induced degeneration and necrosis of hepatic parenchyma, vacuolation, diffusion of Kupffer cells and mononuclear infiltration in the liver of rats.These results agree with the findings of other authors [44,45].The kidneys of AA intoxicated rats demonstrated mono cellular infiltration, degenerated epithelial cells in renal tubules which may be due to the fact that kidney excretes AA and its metabolites [45 -47].Previous facts suggested that AA induced neurotoxicity is associated with central and peripheral nerve terminal damage.This may be due to nerve terminals and cerebellar Purkinje cells damage [44].
Flavonoids are polyphenolic compounds act as scavengers of free radicals by OH groups in their molecular structure which have a property in animal and human models which is helpful for preventing a number of chronic diseases and reduced toxicities [48 -51].PUFA (Polyunsaturated fatty acids) are hydrocarbon chains with two double bonds and lack several hydrogen atoms which are essential for the healthrelated benefits and proper functioning of the human body [52,53].Their deficiency leads to growth retardation, infertility, skin problem, the function of the mitochondria and kidney regeneration [53].It maintains the cell membranes and control metabolism of cells which strengthen the body's natural defenses along with suppressing inflammation [54].It is noteworthy that, in the AA supplemented with LA, enhanced hepatic, renal and cerebral antioxidant enzyme activities when compared with the AA administered group.As a result, LA may have a protective role against ROS mediated damage, thereby causes prevention against lipid peroxidation.Thus, it seems quite reasonable to say that LA was effective for preventing hepatic, renal and cerebral damage which may be due to its free radical scavenging activity.These results provide further evidence for the antioxidant properties of dietary flavonoids as chemopreventive agents in AA toxicity as it has a hydroxyl group.
Apart from the animal studies, toxic consequences of acrylamide on human beings or its presence in human tissues samples and junk foods may also be investigated in the future to correlate the multi-organ dysfunction and acrylamide exposure.In general, disadvantages for antioxidant supplementation are scanty and excess antioxidant supplements may cause metabolic disorders.However, lesser doses of antioxidant have been used and evaluated in this studyFurther, investigations are also necessary to evaluate the therapeutic potential and mechanism of LA for the protection against AA toxicity.

ETHICS APPROVAL AND CONSENT TO PARTICIPATE
The experimentation on animals had been conducted with prior approval of Institutional Ethics Committee (No. IAEC/ JU/2012/04 and date of approval 16th May 2012).

HUMAN AND ANIMAL RIGHTS
No humans were used for studies that are the basis of this research.All applicable national and institutional (CPCSEA) guidelines for the care and use of animal were followed.

CONSENT FOR PUBLICATION
Not applicable.

Table 2 . Influence of vitamin F against AA induced toxicity on lipid profile and kidney function markers of rats.
@Values are mean + S.E., N = 6.#P < 0.05 vs control group, * P < 0.05 vs AA administered group.ANOVA (F values) @ = Significant, ns = Non significant at 5% level.42Wistar

Table 3 . Influence of vitamin F against AA induced toxicity on acetyl cholinesterase activity.
ns= Non significant at 5% level.

Table 5 . Influence of vitamin F against AA induced toxicity on oxidative stress markers in rats. Superoxide Dismutase (µ/min/mg Protein) Catalase (µmol H2O2/min/mg Protein)
ns= Non significant at 5% level.