Siamois Polyphenols as Circulating Endogenous Stem Cell Regulators: Primordial Sources for Repair and Regeneration of Tissue in vivo
Nutthapong Moonkum1, 2, Apijit Chaichana1, Poladate Kantakhum1, Chawanakorn Malimart1, Chaliow Piyachon1, Nopawan Chananpanich3, Samlee Mankhetkorn1, *
Circulating endogenous stem cells (CESs) are an accessible source of adult stem cells and are readily found in all adult tissues.
The objective of this study was to characterize and evaluate the role of CESs in repair and regeneration of tissue in vivo.
The CESs were obtained from healthy (aged 5-10 years (n=5); 22-25 years (n=11); 60-88 years (n=11)) and degenerative disease groups (before and after treatment using Siamois polyphenols (n=4)) and were cultured in conventional and on 3D-nanofibrous scaffold culture. Cells were analyzed under a high-resolution microscope. The proteomic profiling of whole cell and plasma proteins was performed.
CESs exhibited growth and differentiation patterns independently of the sex and age of the donors, while the cellular kinetics was strictly found in homeostasis and can originate specific tissues in 3D culture. The results allow the proposed diagram of repair and regeneration showing the interaction among genes and proteins. It was clearly shown that at least 5 proteins including leucine-rich repeat-containing protein, cardiotrophin-like cytokine factor 1, sushi repeat-containing protein precursor, superoxide dismutase [Mn] and serpin A12 might be potential biomarkers for repair and regeneration processes in both normal and degenerative groups. Siamois polyphenols efficiently regulate the CESs and upregulate the five biomarkers.
The overall results indicated that CESs are crucial sources of cells for repairing and regenerating tissue. Siamois polyphenols might be considered as promising molecules which play important roles in the regulation of CESs for overcoming the degeneration of tissue.
Correspondence: Address correspondence to this author at the Laboratory of Physical Chemistry, Molecular and Cellular Biology; Center of Excellence for Molecular Imaing, Cancer Research and Treatment Center, 234 M 4, Pameang, Subdistrict, Doi Saket, Chiang Mai 50220, Thailand; Tel: +66813172282; E-mails: firstname.lastname@example.org; email@example.com