Biomarkers of Oxidative Stress and Inflammation in Chagasic Myocardiopathy
Gerrard Gabriela1, Martí María Belén1, Diviani Romina1, Ceruti María Jose1, Lioi Susana1, Beloscar Juan2, D´Arrigo Mabel1, *
The fact that only part of the population that lives in endemic areas gets Chagas disease and that only some of the patients with chronic infection develop symptoms, supports the importance of investigating the factors of each host in the susceptibility and the development of the disease. Chronic pathological processes and progressive inflammation lead to alterations in the cellular antioxidant status. This imbalance would contribute to the destruction of the parasite and would be related to the cardiac damage observed in patients with chagasic cardiomyopathy. The objective of the present study was to determine the plasma activity of oxidative stress and inflammatory biomarkers: SOD, CAT, GPx, TBARS and TNF-α in chagasic patients with and without cardiomyopathy and healthy individuals. The aim of the present study is to demonstrate the predisposition to severe forms of chagasic heart disease by quantifying the biomarkers mentioned in blood from the study population. The results show significant differences in the enzymatic activities in the different groups of patients, which would mean at the cellular level, an alteration of the antioxidant capacity. Contrary to what we expected (a depletion of these enzymes), patients show an increase in antioxidant activity, that is, they respond to the generation of free radicals. The same trend is observed in the case of TBARS that are elevated in the case of chagasic patients, indicating a high degree of lipid peroxidation and oxidative damage. Regarding TNF-α levels, we found statistically significant differences, which show an active and chronic inflammatory state in these patients. Although we have found significant differences between the CN group and the other groups of patients, we should indicate that between the MCC and ECsinMCC groups, the results obtained did not show marked differences. This is important since it has been shown that patients infected with Tc have a marked antioxidant potential and are able to respond to the oxidative stress induced by the parasite, although this would not be decisive in the evolution of the disease.
Correspondence: Address correspondence to this author at the Área Química Analítica Clínica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santaa Fe, Argentina; Tel: +54 341 4804592; E-mails: firstname.lastname@example.org